![]() Thus, k cat is sometimes called the turnover number. k cat measures the number of substrate molecules turned over per enzyme molecule per second. K cat gives a direct measure of the catalytic production of product under optimum conditions (saturated enzyme). An experiment in which we measure kinetics at high levels of substrate is called a saturation kinetics experiment. These conditions are called “saturation”: the enzyme is full. Why does the rate simplify this way under these conditions?.If is very large compared to K M, what is the rate (V max)?.Remember, the rate reaches its maximum at high substrate levels, when all enzyme is busy churning out products and no enzymes are empty. ![]()
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